Review



mouse monoclonal anti capzb ab  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology mouse monoclonal anti capzb ab
    (A) Btbd3-interacting proteins are enriched in cytoskeleton proteins. Left: A silver staining gel of total lysates of HT-22 cells with or without expressing Btbd3-Flag subjected to immunoprecipitation (IP) with Flag beads, followed by SDS-PAGE. In-gel digestion was then performed, followed by mass spectrometry analysis. Right: Molecular function analysis of Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (B) N-acetylated Btbd3-interacting proteins. Pie charts show proteins associated with Btbd3 depending on Naa10 and Btbd3 N-acetylation. Flag-bead pull-down experiments in WT HT-22 cells expressing Btbd3 WT -Flag or Btbd3 V1P -Flag or in Naa10-KO HT-22 cells expressing Btbd3 WT -Flag were performed. The list of proteins in each group is shown in Table S3. Bottom: Molecular function analysis of 13 N-acetylated Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (C) Btbd3 binding to <t>CapZb</t> and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag IP was performed in WT or Naa10-KO HT-22 cells expressing the indicated proteins, followed by western blotting. Input comprises 10% of the total cell lysates. (D) Naa10 KO reduces the association of CapZb and F-actin. (E) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (F) Btbd3 N-α-acetylation enhances CapZb association with F-actin. (D-F) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 cells expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (D) , 4 (E) and 5 (F) are shown. (C-F) Repeats of western blotting experiments from figure 3.
    Mouse Monoclonal Anti Capzb Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti capzb ab/product/Santa Cruz Biotechnology
    Average 93 stars, based on 7 article reviews
    mouse monoclonal anti capzb ab - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Naa10 regulates hippocampal neurite outgrowth via Btbd3 N-α-acetylation-mediated actin dynamics"

    Article Title: Naa10 regulates hippocampal neurite outgrowth via Btbd3 N-α-acetylation-mediated actin dynamics

    Journal: bioRxiv

    doi: 10.1101/2024.05.09.583166

    (A) Btbd3-interacting proteins are enriched in cytoskeleton proteins. Left: A silver staining gel of total lysates of HT-22 cells with or without expressing Btbd3-Flag subjected to immunoprecipitation (IP) with Flag beads, followed by SDS-PAGE. In-gel digestion was then performed, followed by mass spectrometry analysis. Right: Molecular function analysis of Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (B) N-acetylated Btbd3-interacting proteins. Pie charts show proteins associated with Btbd3 depending on Naa10 and Btbd3 N-acetylation. Flag-bead pull-down experiments in WT HT-22 cells expressing Btbd3 WT -Flag or Btbd3 V1P -Flag or in Naa10-KO HT-22 cells expressing Btbd3 WT -Flag were performed. The list of proteins in each group is shown in Table S3. Bottom: Molecular function analysis of 13 N-acetylated Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (C) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag IP was performed in WT or Naa10-KO HT-22 cells expressing the indicated proteins, followed by western blotting. Input comprises 10% of the total cell lysates. (D) Naa10 KO reduces the association of CapZb and F-actin. (E) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (F) Btbd3 N-α-acetylation enhances CapZb association with F-actin. (D-F) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 cells expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (D) , 4 (E) and 5 (F) are shown. (C-F) Repeats of western blotting experiments from figure 3.
    Figure Legend Snippet: (A) Btbd3-interacting proteins are enriched in cytoskeleton proteins. Left: A silver staining gel of total lysates of HT-22 cells with or without expressing Btbd3-Flag subjected to immunoprecipitation (IP) with Flag beads, followed by SDS-PAGE. In-gel digestion was then performed, followed by mass spectrometry analysis. Right: Molecular function analysis of Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (B) N-acetylated Btbd3-interacting proteins. Pie charts show proteins associated with Btbd3 depending on Naa10 and Btbd3 N-acetylation. Flag-bead pull-down experiments in WT HT-22 cells expressing Btbd3 WT -Flag or Btbd3 V1P -Flag or in Naa10-KO HT-22 cells expressing Btbd3 WT -Flag were performed. The list of proteins in each group is shown in Table S3. Bottom: Molecular function analysis of 13 N-acetylated Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (C) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag IP was performed in WT or Naa10-KO HT-22 cells expressing the indicated proteins, followed by western blotting. Input comprises 10% of the total cell lysates. (D) Naa10 KO reduces the association of CapZb and F-actin. (E) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (F) Btbd3 N-α-acetylation enhances CapZb association with F-actin. (D-F) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 cells expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (D) , 4 (E) and 5 (F) are shown. (C-F) Repeats of western blotting experiments from figure 3.

    Techniques Used: Silver Staining, Expressing, Immunoprecipitation, SDS Page, Mass Spectrometry, Binding Assay, Western Blot, Activity Assay

    (A) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag immunoprecipitation (IP) was performed in WT or Naa10-KO HT-22 neurons expressing the indicated proteins, followed by western blotting using antibodies against Flag, CapZb, β-actin, and Naa10. Input represents 10% of total cell lysates. The experiment was replicated twice, with the additional replicate shown in . (B) Naa10 KO reduces the association of CapZb and F-actin. (C) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (D) Btbd3 N-α-acetylation enhances CapZb association with F-actin. Western blotting was performed using Abs against CapZb, β-actin, and Btbd3. (B-D) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 neurons expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (B) , 4 (C) and 5 (D) are shown. The bar charts display the relative ratios of CapZb to β-actin in the F-actin fraction with individual data points and mean ± SD for each group from three independent experiments (see -F for replicates). (B) analyzed by unpaired two-tailed t test. (C-D) analyzed by two-way ANOVA plus Sidak’s post-hoc.
    Figure Legend Snippet: (A) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag immunoprecipitation (IP) was performed in WT or Naa10-KO HT-22 neurons expressing the indicated proteins, followed by western blotting using antibodies against Flag, CapZb, β-actin, and Naa10. Input represents 10% of total cell lysates. The experiment was replicated twice, with the additional replicate shown in . (B) Naa10 KO reduces the association of CapZb and F-actin. (C) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (D) Btbd3 N-α-acetylation enhances CapZb association with F-actin. Western blotting was performed using Abs against CapZb, β-actin, and Btbd3. (B-D) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 neurons expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (B) , 4 (C) and 5 (D) are shown. The bar charts display the relative ratios of CapZb to β-actin in the F-actin fraction with individual data points and mean ± SD for each group from three independent experiments (see -F for replicates). (B) analyzed by unpaired two-tailed t test. (C-D) analyzed by two-way ANOVA plus Sidak’s post-hoc.

    Techniques Used: Binding Assay, Immunoprecipitation, Expressing, Western Blot, Activity Assay, Two Tailed Test



    Similar Products

    mouse monoclonal anti capzb ab  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology mouse monoclonal anti capzb ab
    (A) Btbd3-interacting proteins are enriched in cytoskeleton proteins. Left: A silver staining gel of total lysates of HT-22 cells with or without expressing Btbd3-Flag subjected to immunoprecipitation (IP) with Flag beads, followed by SDS-PAGE. In-gel digestion was then performed, followed by mass spectrometry analysis. Right: Molecular function analysis of Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (B) N-acetylated Btbd3-interacting proteins. Pie charts show proteins associated with Btbd3 depending on Naa10 and Btbd3 N-acetylation. Flag-bead pull-down experiments in WT HT-22 cells expressing Btbd3 WT -Flag or Btbd3 V1P -Flag or in Naa10-KO HT-22 cells expressing Btbd3 WT -Flag were performed. The list of proteins in each group is shown in Table S3. Bottom: Molecular function analysis of 13 N-acetylated Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (C) Btbd3 binding to <t>CapZb</t> and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag IP was performed in WT or Naa10-KO HT-22 cells expressing the indicated proteins, followed by western blotting. Input comprises 10% of the total cell lysates. (D) Naa10 KO reduces the association of CapZb and F-actin. (E) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (F) Btbd3 N-α-acetylation enhances CapZb association with F-actin. (D-F) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 cells expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (D) , 4 (E) and 5 (F) are shown. (C-F) Repeats of western blotting experiments from figure 3.
    Mouse Monoclonal Anti Capzb Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti capzb ab/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    mouse monoclonal anti capzb ab - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Btbd3-interacting proteins are enriched in cytoskeleton proteins. Left: A silver staining gel of total lysates of HT-22 cells with or without expressing Btbd3-Flag subjected to immunoprecipitation (IP) with Flag beads, followed by SDS-PAGE. In-gel digestion was then performed, followed by mass spectrometry analysis. Right: Molecular function analysis of Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (B) N-acetylated Btbd3-interacting proteins. Pie charts show proteins associated with Btbd3 depending on Naa10 and Btbd3 N-acetylation. Flag-bead pull-down experiments in WT HT-22 cells expressing Btbd3 WT -Flag or Btbd3 V1P -Flag or in Naa10-KO HT-22 cells expressing Btbd3 WT -Flag were performed. The list of proteins in each group is shown in Table S3. Bottom: Molecular function analysis of 13 N-acetylated Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (C) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag IP was performed in WT or Naa10-KO HT-22 cells expressing the indicated proteins, followed by western blotting. Input comprises 10% of the total cell lysates. (D) Naa10 KO reduces the association of CapZb and F-actin. (E) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (F) Btbd3 N-α-acetylation enhances CapZb association with F-actin. (D-F) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 cells expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (D) , 4 (E) and 5 (F) are shown. (C-F) Repeats of western blotting experiments from figure 3.

    Journal: bioRxiv

    Article Title: Naa10 regulates hippocampal neurite outgrowth via Btbd3 N-α-acetylation-mediated actin dynamics

    doi: 10.1101/2024.05.09.583166

    Figure Lengend Snippet: (A) Btbd3-interacting proteins are enriched in cytoskeleton proteins. Left: A silver staining gel of total lysates of HT-22 cells with or without expressing Btbd3-Flag subjected to immunoprecipitation (IP) with Flag beads, followed by SDS-PAGE. In-gel digestion was then performed, followed by mass spectrometry analysis. Right: Molecular function analysis of Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (B) N-acetylated Btbd3-interacting proteins. Pie charts show proteins associated with Btbd3 depending on Naa10 and Btbd3 N-acetylation. Flag-bead pull-down experiments in WT HT-22 cells expressing Btbd3 WT -Flag or Btbd3 V1P -Flag or in Naa10-KO HT-22 cells expressing Btbd3 WT -Flag were performed. The list of proteins in each group is shown in Table S3. Bottom: Molecular function analysis of 13 N-acetylated Btbd3-interacting proteins analyzed using the online tool THE GENE ONTOLOGY RESOURCE. (C) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag IP was performed in WT or Naa10-KO HT-22 cells expressing the indicated proteins, followed by western blotting. Input comprises 10% of the total cell lysates. (D) Naa10 KO reduces the association of CapZb and F-actin. (E) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (F) Btbd3 N-α-acetylation enhances CapZb association with F-actin. (D-F) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 cells expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (D) , 4 (E) and 5 (F) are shown. (C-F) Repeats of western blotting experiments from figure 3.

    Article Snippet: For western blotting, the following Abs were used: mouse monoclonal anti-Naa10 Ab (sc-373920, Santa Cruz), mouse monoclonal anti-actin Ab (MAB1501, Merck Millipore), anti-α-tubulin Ab (T5168, Sigma), mouse monoclonal anti-Flag (M2) (F3165, Sigma), mouse monoclonal anti-Btbd3 Ab (TA808669S, OriGene), and mouse monoclonal anti-CapZb Ab (sc-136502, Santa Cruz).

    Techniques: Silver Staining, Expressing, Immunoprecipitation, SDS Page, Mass Spectrometry, Binding Assay, Western Blot, Activity Assay

    (A) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag immunoprecipitation (IP) was performed in WT or Naa10-KO HT-22 neurons expressing the indicated proteins, followed by western blotting using antibodies against Flag, CapZb, β-actin, and Naa10. Input represents 10% of total cell lysates. The experiment was replicated twice, with the additional replicate shown in . (B) Naa10 KO reduces the association of CapZb and F-actin. (C) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (D) Btbd3 N-α-acetylation enhances CapZb association with F-actin. Western blotting was performed using Abs against CapZb, β-actin, and Btbd3. (B-D) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 neurons expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (B) , 4 (C) and 5 (D) are shown. The bar charts display the relative ratios of CapZb to β-actin in the F-actin fraction with individual data points and mean ± SD for each group from three independent experiments (see -F for replicates). (B) analyzed by unpaired two-tailed t test. (C-D) analyzed by two-way ANOVA plus Sidak’s post-hoc.

    Journal: bioRxiv

    Article Title: Naa10 regulates hippocampal neurite outgrowth via Btbd3 N-α-acetylation-mediated actin dynamics

    doi: 10.1101/2024.05.09.583166

    Figure Lengend Snippet: (A) Btbd3 binding to CapZb and β-actin depends on Naa10 and Btbd3 N-α-acetylation. Btbd3-Flag immunoprecipitation (IP) was performed in WT or Naa10-KO HT-22 neurons expressing the indicated proteins, followed by western blotting using antibodies against Flag, CapZb, β-actin, and Naa10. Input represents 10% of total cell lysates. The experiment was replicated twice, with the additional replicate shown in . (B) Naa10 KO reduces the association of CapZb and F-actin. (C) Acetyltransferase activity of Naa10 promotes CapZb association with F-actin. (D) Btbd3 N-α-acetylation enhances CapZb association with F-actin. Western blotting was performed using Abs against CapZb, β-actin, and Btbd3. (B-D) Total cell lysates of WT, Naa10-KO or Btbd3-KO HT-22 neurons expressing the indicated proteins were separated into globular actin (G-actin) and F-actin fractions, followed by western blotting. The bands of CapZb and β-actin in F-actin fraction were quantified using ImageJ. The relative ratios of CapZb to β-actin of each lane compared to lanes 3 (B) , 4 (C) and 5 (D) are shown. The bar charts display the relative ratios of CapZb to β-actin in the F-actin fraction with individual data points and mean ± SD for each group from three independent experiments (see -F for replicates). (B) analyzed by unpaired two-tailed t test. (C-D) analyzed by two-way ANOVA plus Sidak’s post-hoc.

    Article Snippet: For western blotting, the following Abs were used: mouse monoclonal anti-Naa10 Ab (sc-373920, Santa Cruz), mouse monoclonal anti-actin Ab (MAB1501, Merck Millipore), anti-α-tubulin Ab (T5168, Sigma), mouse monoclonal anti-Flag (M2) (F3165, Sigma), mouse monoclonal anti-Btbd3 Ab (TA808669S, OriGene), and mouse monoclonal anti-CapZb Ab (sc-136502, Santa Cruz).

    Techniques: Binding Assay, Immunoprecipitation, Expressing, Western Blot, Activity Assay, Two Tailed Test